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Recombinant Human Noggin(Cat. No.:CB89)
DS
COA
价格:
规格:
10μg
50μg
500μg
1mg
数量:
-
1
+
产品描述
Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.
蛋白编号
Q13253
产品别称
Noggin; NOG
分子量
23 KDa
表观分子量
25-33 KDa, reducing conditions
剂型描述
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4.
内毒素
< 0.01 EU/µg as determined by LAL test.
产品稳定性
Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Noggin
纯度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
纯度-SEC-HPLC
Greater than 95% as determined by SEC-HPLC. (QC verified)
生物活性-Cell Based Assay
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 10-100 ng/ml in the presence of 300 ng/mL of Recombinant Human BMP‑4(Cat.NO.:CR93)(QC Verified).
The activity of Recombinant Human Noggin Protein(Cat. No.:CB89) was compared to another commercially available product.
Human breast cancer organoids were cultured with Wnt3a(Cat#C22R),Noggin (Cat#CB89),R-spondin 3 (Cat#C18C),KGF(Cat#CH73), FGF-10(Cat#CR11),EGF (Cat#C029)and NRG1-beta 1(Cat#753). DAPI (blue), Ki67(red) and ER(green).
复溶
  • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
  • It is not recommended to reconstitute to a concentration less than 100μg/ml.
  • Dissolve the lyophilized protein in distilled water.
  • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
运输条件
  • The product is shipped at ambient temperature.
  • Upon receipt, store it immediately at the temperature listed below.
保存条件
  • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
  • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
  • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
产品背景
Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.
仅供科研或生产使用,不可直接应用于人体。
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